Thermo Scientific MAbPac SCX-10 - Cation-Exchange Column for Monoclonal Antibody Analysis

The MAbPac SCX-10 is a strong cation-exchange (SCX) column specifically designed for charge variant analysis of monoclonal antibodies (mAbs) and other biopharmaceutical proteins. It provides high-resolution separation of acidic, main, and basic charge variants critical for biotherapeutic characterization.

Key Specifications:

• Manufacturer: Thermo Fisher Scientific
• Column Type: Strong cation exchange (SCX)
• Application: Monoclonal antibody charge variant analysis
• Substrate: Non-porous polymer beads
• Dimensions: 4 x 250 mm
• Part Number: 074631

Why Choose MAbPac SCX-10?

The MAbPac SCX-10 column is purpose-built for the biopharmaceutical industry. Unlike general-purpose SCX columns, the MAbPac SCX-10 uses optimized surface chemistry and particle characteristics specifically for large biomolecule separations.

The non-porous particle design eliminates pore-related band broadening, delivering sharp peaks and high resolution between closely related charge variants. The column provides excellent protein recovery, minimizing sample loss during analysis.

Applications:

• Monoclonal antibody (mAb) charge variant profiling
• Biosimilar comparability studies
• Antibody-drug conjugate (ADC) characterization
• Fc fusion protein charge heterogeneity analysis
• Biopharmaceutical lot release and stability testing
• Process development and cell line optimization

Typical Method:

• Mobile Phase A: 20 mM MES, pH 5.6
• Mobile Phase B: 20 mM MES + 500 mM NaCl, pH 5.6
• Gradient: 0–100% B over 30 minutes
• Detection: UV at 280 nm
• Temperature: 25–30°C

Compatible with Thermo Scientific Vanquish, UltiMate 3000, and other bio-compatible UHPLC/HPLC systems.

Order from Eke Lab Shop. Contact us for biopharmaceutical column support.