Merck LiChrospher RP-8 - C8 Reversed-Phase Column for Less Hydrophobic Separations

The LiChrospher RP-8 is a C8 (octylsilane) reversed-phase HPLC column manufactured by Merck (Sigma-Aldrich). It provides shorter alkyl chain length compared to C18 columns, resulting in weaker hydrophobic interactions and shorter retention times for moderately hydrophobic analytes.

Key Specifications:

• Manufacturer: Merck (Sigma-Aldrich)
• Phase: RP-8 (C8, octylsilane)
• Pore Size: 100 Å
• Particle Size: 5 µm
• Substrate: LiChrospher spherical silica
• Endcapped: Yes
• USP Classification: L7
• Mode: Reversed Phase

Why Choose LiChrospher RP-8 Over C18?

C8 columns like the LiChrospher RP-8 are the preferred choice when C18 retention is too strong for your target analytes. The shorter octyl chain provides faster elution of hydrophobic compounds, enabling shorter run times and lower organic solvent consumption.

C8 phases also provide different selectivity compared to C18, making them valuable as an orthogonal method during method development. If your USP or EP method specifies a L7 (C8) stationary phase, the LiChrospher RP-8 is a reliable, pharmacopeia-compliant choice.

Applications:

• USP and EP methods specifying L7 (C8) phases
• Pharmaceutical impurity profiling with moderate hydrophobicity analytes
• Peptide and small protein analysis
• Lipophilic compound analysis requiring shorter retention
• Method development as orthogonal selectivity to C18
• Environmental and food safety screening

Available in multiple dimensions and LiChroCART cartridge format. Compatible with all major HPLC systems.

Order from Eke Lab Shop. Contact us for C8 vs C18 selection guidance and column recommendations.